Bioethanol Production of Pine Merkusii leaf with Select Enzyme using Method (SSF)
Increased fuel demand encourages the search for material sources raw material that can be used to produce fuel as
a source renewable. Therefore, it needs a new innovation to create as well developing renewable energy from waste, its
potential very fulfilling or easy to get around. Bioethanol is a source of energy renewable environmentally friendly from
waste raw materials. Bioethanol (C2H5OH) is one of the fuels that comes from a biological source that can replace
petroleum. Bioethanol can be produced from the fermentation of sugar from source of carbohydrates (starch) using the help
of microorganisms that can obtained from materials containing cellulose. This study was conducted for utilizing the waste of
pine leaf which is very abundant to reduce environmental pollution in this innovation using stems from oyster mushrooms
which is useful as enzyme activation in bioethanol making by method SSF (Simultaneous Scarification and Fermentation) by
converting the polysaccharide compound becomes a monosaccharide compound without turning back to polysaccharide
compounds. This research uses qualitative analysis with GC instruments that show that pine leaves can produce bioethanol
as a substitute for petroleum, as well as quantitative tests using UV-Vis spectrophotometer instrument with optimum yield
on 72-hour time variation.
Index Terms - Bioethanol, Cellulose, SSF Method, Qualitative and Quantitative Test.