Paper Title
Identification and Antioxidant Activity of Alamanda Leaf (Allamanda Cathartica l.) Based on Solvent Variations

Abstract
Alamanda (Allamanda cathartica L.) included in Apocynaceae family. Empirically, people use alamanda leaf as anticancer. The purpose of this study was to determine the chemical content and activity level of antioxidant compounds from alamanda leaf extract (Allamanda cathartica L.) based on solvent variation. Extraction method used was maceration using n-hexane, ethanol and water solvent, and for infuse using water. Determination of antioxidant compound activity from alamanda leaf extract (Allamanda cathartica L.) using DPPH (2,2-diphenyl-1-picrylhydrazil) method. The results showed that alamanda leaf extract was positively contains Alkaloid, flavonoid, phenolic and saponin compounds. While the antioxidant activity of alamanda leaf (Alamandachatartica L) in water extract had strong antioxidant activity with IC50 value of 44,899 μg/mL, ethanol extract had weak antioxidant activity with IC50 value of 106,352 μg/mL, and for n-Hexane extract had very weak antioxidant activity with IC50 value of 164,063 μg/mL. while the quersetin reference standard has an IC50 of 9,112 μg/mL indicating strong antioxidant activity. Index terms - Alamanda leaf (Allamanda catharticaL.), Antioxidant, DPPH