In Vitro Study the Effect of Plant Growth Regulators and Response of Two Varieties of Iraqi Maize
In vitro plant tissue culture study the effect of plant growth regulators and response of two varieties of maize(5018
and Baghdad-3) to callus induction and regeneration using the 2,4-D (0.75, 1.5, 2.25 and 3.0) mg.L-1 to induce callus, the
[BAP (1.0, 2.0, 3.0, and 4.0) mg.L-1 and NAA (0.0, 0.5, 1.0 and 1.5) mg.L-1] to regenerate plantlet from induced callus and
the IBA (0.0, 1.5, 3.0, 4.5 and 6.0) mg.L-1 to reform roots. The highest percentage to callus induction was 78% and the
lowest was 13%. The highest and lowest average wet and dry weights to callus (245.3 and 102.4) mg (27.4 and 11.2) mg
respectively. The highest and lowest average branches shoot number and length (20.0 and 1.8) branches.plant-1 and (11.3 and
2.3) cm respectively. The highest and lowest average root number and length (25.3 and 3.8) root.plant-1 and (6.4 and 2.4) cm
respectively. The results show the presence of significant differences between the two varieties and growth regulators
concentrations where the variety (5018) exhibits higher response.
Index terms- Callus induction,Plant growth regulators, Maize.