Paper Title :Detection of E. Coli O157:H7 in Several Food in Different Areas of Baghdad

Author :Manal .K.Alhadeethy, Elham .E.Alshamary

Article Citation :Manal .K.Alhadeethy ,Elham .E.Alshamary , (2023 ) " Detection of E. Coli O157:H7 in Several Food in Different Areas of Baghdad " , International Journal of Advances in Science, Engineering and Technology(IJASEAT) , pp. 17-21, Volume-11,Issue-2

Abstract : This study was conducted to detect E. coli O157:H7 in some food( minced beef , beef burger , white soft cheese and salads ) from different areas in Baghdad from September 2019 until November 2019 , (SMAC) Medium was used to detect the bacteria and to differentiated it from other Entero bacteriaceae .The number of obtained isolates reached 283 ,231,219 isolation respectively for September , October and November , the results showed rise in the total number of the isolations that were obtained from September comparison with October and November. The sorbitol fermentative isolates percentage reached 85% ,88% and 94% while non sorbitol fermentative isolates reached 11%, 5% and 4% .Isolates were non sorbitol fermentative subjected to cultural and morphological examinations, the isolates appeared in a pale golden color, with a rod shape, G-, moving ,and 42 isolates were non-sorbitol fermentative identified using VITEK-2, one isolate was E.coli O157:H7 that was obtained from the burger with a probability percent of 97% ,and was also identified genetically with PCR using the Escherichia coli O157:H7C1-057,Escherichia coli O157:H7 FRIK944 ,Escherichia coli O157:H7 FRIK2455, Escherichia coli O157:H7 FRIK2069,Escherichia coli O157:H7 FRIK2533, (accuracy of 100%) and with Escherichia coli O157:H7 AR-0427(accuracy of 99%), which was matched with the sequence of O157:H7 strain FS94 also were subjected to latex test a specific test to the strain E. coli O157:H7. Keywords - E. Coli O157:H7, PCR ,Latex Test.

Type : Research paper

Published : Volume-11,Issue-2

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