Paper Title
Comparative Study of Some Biochemical Markers in Seminal Plasma and Serum for Three Groups of Infertility Men Patient
Abstract
High rates of infertility and pollution in Iraq in particular, and in the countries of the region in general, because of
the wars, led to the emergence of many difficulties in the diagnosis of infertility, so the objective of this study was to find out
which methods used to diagnose infertility is better, whether in serum, semen or both.This study determined the main
fertility hormones and some biochemical markers in both blood and seminal plasma by ELISA method, and Total protein
was measured by spectrophotometer.The current study showed the biochemical markers were an approximate match in the
levels of its concentrations in both seminal plasma and blood serum. In addition, there was a significant increase (p<0.05) in
the level of FSH, LH, Prolactin and Total protein in both Azoospermia and Oligospermia in comparison with the control
group (Normospermia) in seminal plasma and serum.whereas there was a significant decrease (p<0.05) in the level of
(Testosterone, Inhibin B, AMH, and Natural α- Gluc) in both Azoospermia and Oligospermia comparison with the control
group in seminal plasma and serum. The correlations of both serum and seminal biochemical markers with seminal plasma
parameters were somewhat similar. BMI has a significant increase (p< 0.05) in both Azoospermia (29.80± 3.68 Kg/m²), and
Oligospermia (27.96± 2.42 Kg/m²) comparison with Normospermia (22.10± 3.09 Kg/m²). In conclusion, estimation of
reproductive hormones in seminal plasma along with serum is a crucial tool in order to better identify and accurate treatment
for male infertility disorders such as impaired spermatogenesis, especially in case of seminal (LH, Inhibin B, and AMH)
were higher than those in serum. As well as high BMI level has a mischievous effect on semen quality and concentrations of
reproductive hormones.
Keywords- BMI, AMH, Inhibin B, NAG.