Paper Title :Chlorogenic Acid Content in Lonicera Japonica Flowering Bud by Quantitative Thin-Layer Chromatography
Author :Chayanon Chaowuttikul, Chanida Palanuvej, Nijsiri Ruangrungsi
Article Citation :Chayanon Chaowuttikul ,Chanida Palanuvej ,Nijsiri Ruangrungsi ,
(2017 ) " Chlorogenic Acid Content in Lonicera Japonica Flowering Bud by Quantitative Thin-Layer Chromatography " ,
International Journal of Management and Applied Science (IJMAS) ,
pp. 12-15,
Volume-3,Issue-10
Abstract : Lonicera japonica Thunb. or Japanese honeysuckle has been widely used in traditional medicine to treat many
ailments. Chlorogenic acid is a main active compound in this plant. Dried L. japonica flowering buds were purchased from 15
various herbal drugstores throughout Thailand. Chlorogenic acid contents were quantitatively analysed by TLC‑densitometry
with winCATS software compared to TLC-image analysis with ImageJ software. The mobile phase for TLC development
consisted of ethyl acetate : formic acid : acetic acid : water (10:1.1:1.1:2.6). Chlorogenic acid content was found to be
2.24±0.50 and 2.09±0.44 g/100 g of crude drug by TLC‑densitometry and TLC-image analysis respectively. There was not
statistically significantly difference between these methods. Consequently, TLC-image analysis could be used as an
alternative method in place of TLC-densitometry.
Index Terms - Chlorogenic acid, Lonicera japonica, quantitative analysis, thin-layer chromatography
Type : Research paper
Published : Volume-3,Issue-10
DOIONLINE NO - IJMAS-IRAJ-DOIONLINE-9760
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Copyright: © Institute of Research and Journals
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Published on 2018-01-03 |
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